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  • - (upbeat music)

  • - Welcome to the Biotechnology Program

  • and your first day of laboratory class.

  • My name is Sarah Leonard,

  • and I'm the Laboratory Manager in the BIT Program.

  • It's my responsibility to go over

  • all the safety protocols every semester

  • to make sure that everyone's on the same page

  • about how to handle our microorganisms

  • and biological agents in a safe manner.

  • You have been given an informed consent sheet

  • that outlines the specific biological agents

  • that you will be working with,

  • how to handle them, and what to do

  • in case of infection or contamination.

  • Here in BIT we have four lab spaces

  • which are each rated up to a biosafety level two.

  • That rating comes with specific requirements

  • for personal protective equipment,

  • lab features, and decontamination.

  • Personal protective equipment is the first defense

  • against anything that you might

  • come in contact with while in the laboratory.

  • In our labs you're required to wear

  • safety glasses, gloves, lab coats,

  • closed-toed shoes, and long pants.

  • Safety glasses protect your eyes against splashes.

  • They also keep you from accidentally rubbing your eyes

  • with unwashed or gloved hands.

  • Safety glasses should be worn at all times

  • while in the laboratory.

  • Do you wear eyeglasses?

  • Try a wide pair.

  • Please make sure to find a pair of safety glasses

  • that work for you.

  • If you can't let me know and we'll help you

  • find a pair that fits.

  • There are gloves in every lab in at least two places.

  • Make sure to wear gloves whenever

  • you are touching your experiments or equipment

  • in the lab, but remember to take them off

  • and wash your hands before leaving the room.

  • If your gloves are contaminated or torn

  • dispose of them right away in the biohazard bins

  • and get a new pair.

  • Don't try to wash them.

  • Gloves are not as expensive as your safety.

  • Lab coats are worn during all activities in the lab.

  • We use disposable lab coats which are replaced

  • every eight weeks of class.

  • They come in a wide range of sizes.

  • Try to find a size that fits for you.

  • Do not wear gloves or lab coats in common areas

  • like the kitchen or the bathroom.

  • The one exception is going in between the labs

  • or getting ice for ice baths.

  • Lab coats are stored in designated drawers

  • at your assigned station.

  • At the end of each lab take off your lab coat

  • and store it in your drawer

  • so that it's there for you to use the next class.

  • If your lab coat is torn or contaminated

  • make sure to dispose of it and get a clean one

  • to use the next time.

  • It is absolutely essential to wear proper shoes in the lab.

  • This means shoes that cover the front

  • and back of your foot.

  • Sneakers are ideal.

  • If you do not wear the right shoes to lab

  • you will be asked to leave.

  • If you have long hair

  • or if you have dangling jewelry or loose clothing,

  • please make sure to tie them back out of the way.

  • This is for your protection

  • as well as your experiment's protection.

  • You don't want to contaminate your experiment

  • or accidentally take your work home with you.

  • There is no food, gum, candy, or drinks in the lab,

  • including water.

  • This also includes snacks that you may have

  • stored in your book bag.

  • Don't let them go through the doorway.

  • We have carts outside the labs

  • where you may store your food during the lab period,

  • or down the hallway there's a kitchen area.

  • You are welcome to store your food

  • in the refrigerator or on the shelves in the kitchen.

  • Cell phones and other personal items

  • are the most common vectors for contamination.

  • We encourage you to use your cell phone

  • to take pictures of your experiments

  • for documentation in your lab reports.

  • But, if you're going to handle a personal item,

  • please take off your gloves and wash your hands.

  • That way you eliminate that possibility of accidentally

  • transferring some sort of contaminant

  • to your cell phone, which you might later

  • transfer to yourself when you go to use your cell phone.

  • You also want to avoid touching your face,

  • and that also includes not messing

  • with your contacts or biting your fingernails.

  • Please make sure to store your bags

  • either in the provided cubbies or in the kick-outs

  • up underneath your bench tops.

  • This ensures that no one trips

  • over a loose backpack strap.

  • We don't want anyone ending up on the floor.

  • Take a look around you.

  • Familiarize yourself with where things are in the lab.

  • Make note of where to find the eye wash,

  • safety shower, the hand-washing sinks,

  • fire extinguisher, and emergency exits.

  • Every lab is equipped with an eye wash

  • and a safety shower.

  • This is case you should have a splash in your eye

  • or in the case of the safety shower, on your body.

  • They are tested weekly to ensure

  • that they run properly and that there is nothing

  • caught up in the water lines.

  • The showers are for large spills on the person

  • and the eye washes are to rinse out small

  • spills or particulates in your eyes.

  • Please note, though, there are no drains

  • around these, so they are for emergency use only.

  • We try to minimize the use of sharps

  • in our classes, but you may still

  • come across some broken glass or plastic.

  • If you do have a sharp, your TA will help you

  • dispose of it in the red biohazard containers

  • that say "Sharps" on them.

  • Do not throw away sharp objects in the normal trash cans.

  • If there is a spill please notify your TA

  • or myself or one of your instructors,

  • so that we can help you clean it up properly.

  • If it's just a small spill you can clean that up

  • with some paper towels and disinfectant,

  • but still let your TA or instructor know

  • so that we can make sure everything

  • is cleaned up as it should be.

  • All contaminated materials, including anything

  • biological, should be disposed of

  • in a biohazard waste container.

  • Every lab has a large red biohazard waste container

  • for larger materials.

  • This includes things like agar plates.

  • On every benchtop is a smaller orange biohazard container.

  • This can be used to dispose of small objects

  • like your pipette tips,

  • micro-centrifuge tubes or chem wipes.

  • You can use this during your lab

  • and at the end of lab you can close the lid

  • and leave it there as long as your bag

  • is less than half full.

  • If your bag is more than half full,

  • please take the inner plastic bag out,

  • throw it in the big red biohazard container,

  • and replace it with a clean bag

  • that your TA can help you find.

  • You're expected to use the provided pens

  • for marking things and taking notes.

  • These pens are recognizable because

  • they have the same color scheme

  • as the TA and Lab Instructor coats of tie-dye.

  • If you are wearing gloves and touching

  • your experiment only use these pens.

  • Writing utensils are a personal item

  • just like your cell phone,

  • and pens are also common vectors for contamination.

  • So while you're in the lab use the provided pens

  • and please leave them here at the end of the day.

  • Labeling might seem like an odd topic

  • for a safety presentation but over the course

  • of your class you're gonna be creating

  • samples that you don't wanna lose.

  • Be mindful that we have up to six sections

  • of one lab running at any time,

  • so if you do not label your containers properly

  • it could get mixed up with someone else's and be lost.

  • Your whole semester's worth of work will be gone with it.

  • So when you store your experiments

  • in containers, label them carefully.

  • Make sure to include your initials,

  • the date, your lab section, and any other descriptive

  • information you can give about the contents.

  • In many of our classes we use the UV light

  • to see tagged proteins.

  • Exposure to UV light can cause skin cancer.

  • You can protect yourself by covering up

  • exposed skin and wearing your safety glasses

  • whenever using the UV light.

  • Each machine has a UV shield that should be used

  • when the light is turned on.

  • As an option we also have full face masks

  • available for students, although they are not required.

  • There are seven steps you need to take

  • before you leave lab at the end of the day.

  • First, disinfect your bench.

  • Each lab has a disinfectant called Decon Conflikt.

  • Spray it on your benchtop and let it sit there

  • for three to five minutes.

  • Then spray ethanol over the same area

  • and use a paper towel to wipe off

  • the disinfectant residue.

  • Turn off all equipment and unplug

  • everything but the laptops.

  • Unplugging equipment reduces their potential

  • as fire hazards.

  • Empty your benchtop biohazard bag

  • if it's over half full.

  • If it's less than half full

  • you may leave it there for the next person

  • to come in and use.

  • Take off your lab coat and store it

  • in your designated drawer, or, if it's contaminated

  • or torn, you may dispose of it

  • and get a new one for the next lab.

  • Remove your gloves, dispose of them

  • in the biohazard bins, and thoroughly wash your hands

  • before leaving the laboratory.

  • If there is an emergency during one of our labs,

  • stay calm and make your way

  • to the nearest exit.

  • There are two evacuation exits on this floor,

  • one on each end of the hallway.

  • There is one stairwell next to the autoclave room

  • which leads out to the back of the building.

  • Consider this the secondary exit.

  • If you are able, we prefer that you use

  • the front stairwell by the elevators

  • and rest rooms.

  • We will use the front exit for meeting purposes.

  • If a fire alarm sounds, stop your work

  • and remove your PPE.

  • Leave your gloves and lab coat

  • at your station, exit the building

  • using one of the two evacuation routes,

  • and meet in front of Jordan Hall.

  • Try to find your instructor or TA

  • so that they can take roll and ensure

  • that everyone made it out of the building safely.

  • If you are concerned about your physical ability

  • to leave the building during an emergency,

  • please come see me and we will discuss

  • a personal safety plan for you.

  • If you have any questions about your safety

  • in the lab, please come see me in my office

  • in room 6119.

  • Or ask your TA or instructor and we will make sure

  • to get your questions answered.

  • Thanks for watching, and from all of us

  • here at BIT we hope you guys have

  • a fun and safe semester.

- (upbeat music)

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B1 中級

ラボ安全トレーニング(BSL-2 (Lab Safety Training (BSL-2))

  • 33 1
    郭玉梅 に公開 2021 年 01 月 14 日
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