Name: 15.PKとNRPシンターゼ1 (15. PK and NRP Synthases 1)
Uploaded: 2021-01-14T10:26:51.000Z
Duration: 50 min 1 s
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ELIZABETH NOLAN: --by talking about ClpX.

And then we're going to move into module 4--

which is the last module before spring break--

on synthases and assembly line biosynthesis.

So basically last time, where we left off is,

done to look at denaturation, translocation,

And closed with a question about what actually is going on

in ClpX with this ATP binding and hydrolysis

substrates to be unfolded and translocated

And I left you just with the statement that although we

think about ClpX as this hexamer that has six identical

subunits, what studies have shown is that there's some

inherent asymmetry within this AAA+ ATPase.

And that's what we're going to talk about a little bit.

So this is just a slide from a few lectures ago

that's showing the top view and side view of ClpX

highlight how complicated these machines are

comes from whether or not each ClpX subunit

And so basically, from looking at many different crystal

structures, what can be done is that the ClpX subunits

can be divided into two different types based

want to first think about the ClpX domain organization.

And if we just think about this, what ClpX has is

that's called the large domain and then followed

So 633 amino acids, just to give you a sense of size,

and what's observed from many different crystal structures.

And so these two different types of subunit

have been described as loadable and unloadable,

and that depends on whether or not nucleotide is bound.

just thinking about the large and small domains,

And we have this region that's called a hinge.

And so the other type is described as unloadable.

nucleotide when in this unloadable conformation.

So what's been found from looking at many crystal

structures is that within the ClpX hexamer,

there's an arrangement of these loadable and unloadable

plus two unloadable subunits arranged with about

So there's some asymmetry in the subunits.

And so also from these crystal structures,

don't see with just these cartoons of the 6-mer.

So we can learn about how subunits interact, of course,

and consider how these subunits interact, what we find

or sorry, the small domain of one ClpX subunit interacts

with the large domain of the adjacent ClpX subunit.

Basically, if we consider a large domain--

the small domain and then the large domain

字幕リスト動画再生

15.PKとNRPシンターゼ1 (15. PK and NRP Synthases 1)

structure

instance

audience

bacteria