Name: #第07回 ケビン・アハーンのBB450/550のための生化学タンパク質特性評価講義 (#07 Biochemistry Protein Characterization Lecture for Kevin Ahern's BB 450/550)
Uploaded: 2021-01-14T05:20:27.000Z
Duration: 51 min 27 s
Description: VoiceTubeの動画で発音を聞きながら英語表現を覚えよう！学べる英語：

Another Monday beckons, another week beckons.

Kevin Ahern: Are we talking about football here?

and I think that's a really phenomenal technology.

us to do amazingly complex analyses of cells.

And if we have cells that have different experiences

one being a tumor cell, one not being a tumor cell,

one not being treated with a drug, one being starved,

the other not being starved, et cetera, et cetera

how these changes occur inside of the cells.

Several students after the class asked me if there were

libraries of gels that were out there that are cells

But many laboratories will actually do their own

side-by-side comparison because one of the things

that you see is the reproducibility is not 100% the same,

you're a little bit more able to compare them.

But, yes, there are libraries of such things out there.

And I just realized, I haven't checked the camera

to make sure it's properly on the screen.

There's nothing worse than looking at your video afterwards

and you see you had it about halfway on screen

And you guys like that about as much as I do,

One of the things I skipped over in getting to tell you

about 2D gel electrophoresis was to tell you about

So that's how I'm going to start the lecture today,

telling you how gel electrophoresis works

two different types of gel electrophoresis.

separating DNA by agarose gel electrophoresis.

Agarose is, and there's the word right there

agarose gel electrophoresis is a technique.

Your book used to have a figure and then they took that

away from me, so I don't have the figure out for it.

very much the same as polyacrylamide gel electrophoresis.

So let me just show you what that looks like.

Agarose gel electrophoresis is what we use

We can also separate fragments of RNA with it.

We do not use agarose gel electrophoresis to separate proteins,

and you'll see why that's the case in just a little bit.

The first reason, though, that we don't use it to separate

proteins is that nucleic acids are way bigger than proteins.

The biggest molecules in the cell are DNA molecules, by far.

Proteins don't even come close in terms of size.

What the agarose provides, in the case of DNA separations,

in the case of protein separations, are a matrix.

sort of like it's schematically shown here.

The matrix is a series of strands or connected

The support is to support the liquid of the buffer.

So just like we could take a mix of Jello

when it cools down, it forms a solid support based

on what was in there, so, too, can we do with materials

for the gel, the difference being, in the case of a gel,

that these strands that provide the support will provide

And I'll show you how that happens, okay?

Agarose has bigger holes than polyacrylamide does.

字幕リスト動画再生

#第07回ケビン・アハーンのBB450/550のための生化学タンパク質特性評価講義 (#07 Biochemistry Protein Characterization Lecture for Kevin Ahern's BB 450/550)

specific

technique

determine

immune

#第07回 ケビン・アハーンのBB450/550のための生化学タンパク質特性評価講義 (#07 Biochemistry Protein Characterization Lecture for Kevin Ahern's BB 450/550)

specific

technique

determine

immune

#第07回ケビン・アハーンのBB450/550のための生化学タンパク質特性評価講義 (#07 Biochemistry Protein Characterization Lecture for Kevin Ahern's BB 450/550)