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Here’s how a spectrophotometer works. A lamp provides the source of light. The beam
of light strikes the diffraction grating, which works like a prism and separates the
light into its component wavelengths. The grating is rotated so that only a specific
wavelength of light reaches the exit slit. Then the light interacts with the sample.
From this point, the detector measures the transmittance and absorbance of the sample.
Transmittance refers to the amount of light that passes completely through the sample
and strikes the detector. Absorbance is a measurement of light that is absorbed by the
sample. The detector senses the light being transmitted through the sample and converts
this information into a digital display.