Name: クロマトグラフィーの基礎 (Basics of Chromatography)
Uploaded: 2021-01-14T06:03:34.000Z
Duration: 9 min 16 s
Description: VoiceTubeの動画で発音を聞きながら英語表現を覚えよう！学べる英語：

down with a black pen on a piece of paper, then

様態の副詞

accidentally smudged the paper with water?

Well, what happens is that you might see the black ink

start to smudge and start to see some colors that

aren't black at all, maybe some dark blues and some darker

This is because black dye is actually made out

of a bunch of different other dyes and different components.

And what you've just witnessed is a basic example

Chromatography involves taking some kind of mixture

and using either solid or liquid to separate it out

There are many different kinds of chromatography,

but they all rely on having a mobile phase

In paper chromatography, the stationary phase is the paper.

or actually, any kind of container will do.

But in this, you'll be putting in your mobile phase.

And your mobile phase can be some kind of solvent.

Now that you have this, you pour a small amount of solvent.

Again, not too much, because you don't want this to already

be above the level of your spot, or else it will just all bleed.

this pink solvent will travel up the piece of paper.

And as it does that, it'll actually take some of the dyes

And let's see what happens at a few different time points.

This implies that it's composed of two different components.

However, if this were a very complex mixture,

you could even see five, six, or a lot of other spots.

If you wait even longer, this is what you'll see next.

You'll see that the spots will continue traveling even farther

up the plate, and the separation between them,

is that whatever was in the green spot originally

That means that it was pretty attracted to that pink solvent

Whereas the yellow spot didn't move quite as much,

This competition between the stationary phase

and the mobile phase pulling at the components

occurs in all different kinds of chromatography.

So let's try to lay this information out in a table.

We've talked about how for paper chromatography,

the stationary phase is a solid; the mobile phase

and they're separating it based on polarity,

meaning how attracted it is to the paper versus the solvent,

We'll see that all the spots on this table

of having a piece of paper, you have a glass slide that

This is a great preparative tool that is commonly

To remind you that these two are related, what I've drawn here

is a little beaker with a small either plate or piece

And the arrow shows that the solvent is traveling up

The next kinds of chromatography we'll be going over

And you fill it with some kind of packing material

You can lower your sample that you want to separate out,